Leptospirosis dan Pengembangan Metode Deteksi Leptospirosis Pada Sapi

Wida Wahidah Mubarokah, Roeswandono Wirjaatmadja, Yos Adi Prakoso, Asih Rahayu, M. Dzaki Wiranda Pratama

Abstract


ABSTRAK
Leptospirosis merupakan penyakit zoonosis yang disebabkan oleh Leptospira sp. Kejadian leptospirosis di Indonesia masih belum menjadi perhatian utama pemangku kebijakan. Hal ini disebabkan karena leptospirosis umumnya tidak menimbulkan gejala klinis yang signifikan pada ternak. Namun, masih diduga kuat bahwa leptospirosis menimbulkan penurunan produksi dan kerugian ekonomi. Selain itu potensi zoonosis yang ditimbulkan harus menjadi perhatian utama. Metode deteksi leptospirosis saat ini masih terbatas pada microscopic agglutination test (MAT). Metode uji ini masih ditemukan banyak kekurangan sehingga seiring waktu dilakukan pengembangan metode lain, seperti histopatologi, enzyme-linked immunosorbent assays (ELISA) maupun reverse-trancriptase polymerase chain reaction (RT-PCR). Sehingga pemahaman tentang metode pengujian yang akurat untuk leptospirosis harus ditingkatkan. Hal ini terkait dengan teknik diagnostika dalam pencegahan, pengendalian, dan pemberantasan leptospirosis pada ternak di Indonesia.

Kata kunci: histopatologi, leptospirosis, MAT, RT-PCR, zoonosis.

 

ABSTRACT
Leptospirosis is a zoonosis disease that caused by Leptospira sp. The evidence of leptospirosis in Indonesia still not becoming the major of concern of the government in policies developing. It is caused by the leptospirosis clinical signs among the livestock generate asymptomatically. However, leptospirosis is suspected causes the decrease of production and economic losses. In addition, the potency of zoonosis must be came a major concern. The detection method of leptospirosis is still using microscopic agglutination test (MAT). This method is found have several limitations compared to enzyme –linked immunosorbent assays (ELISA) and reverse-transcriptase polymerase chain reaction (RT-PCR). The understanding regarding the accurate detection methods for leptospirosis should be increased. It is related to the diagnostic method on prevention, control, and eradication of leptospirosis among the livestock in Indonesia.

Keywords: histopathology, leptospirosis, MAT, RT-PCR, zoonosis.


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References


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